anti porcine cd25 primary antibody  (Bio-Rad)

Journal: Frontiers in Immunology

Article Title: Sexual Dimorphism in Immune Development and in Response to Nutritional Intervention in Neonatal Piglets

doi: 10.3389/fimmu.2019.02705

Figure Lengend Snippet: Representation of 3 color fluorescence immunohistology of CD4 (red), CD25 (blue), Foxp3 (green), and CD4CD25 (magenta) positive staining in the distal jejunum lamina propria of a 28 day old female and male control (no dietary supplementation) piglets (A) . Bar = 10 μm. Quantitative analysis (log transformed) of CD4 + CD25 + Foxp3 + cells/mm 2 of distal jejunum lamina propria in 28 day old piglets by treatment (unsupplemented control, inulin or starch supplemented) and by sex (females = black bars, males = white bars) (B) . Ten individual images were analyzed for each sample. * p < 0.05; ** p < 0.01; *** p < 0.001. Error bars SEM ( n = 5/sex/treatment group).

Article Snippet: The following monoclonal antibodies were used: anti-porcine CD4 (clone MIL17, BioRad); anti-porcine CD172/Sirp-α (clone 74-22-15 Cambridge Bioscience), anti-porcine intestinal capillary endothelium (clone MIL11, Bio-Rad), anti-porcine MHC class II DR (clone MSA-3, BioRad), Rat anti-mouse Foxp3 (clone FJK-16s, ThermoFisher), anti-porcine CD25 (clone K231.3B2, BioRad), rabbit anti-mouse Zona Occludin-1 (ZO-1, clone 61-7300, BioRad), anti-porcine CD45 (clone k252-1e4, BioRad), and mouse anti-rat E-cadherin (clone DECMA-1, AbCam).

Techniques: Fluorescence, Staining, Transformation Assay

Journal: BMC Veterinary Research

Article Title: In vitro immune responses of porcine alveolar macrophages reflect host immune responses against porcine reproductive and respiratory syndrome viruses

doi: 10.1186/s12917-018-1675-x

Figure Lengend Snippet: Frequency of immune cells in PBMCs derived from PRRSV-infected pigs. a Activated CD4 + T cells (CD4 + CD25 + Foxp3 − ), b type I helper T cells (CD4 + IFN-γ + ), c cytotoxic T cells (CD8 + IFN-γ + ), d total PBMCs expressing IFN-γ + and e T Regs (CD4 + CD25 + Foxp3 + ). Each bar represents the average percentage of immune cells from 5 pigs ± SEM. Bars showing different letters represent values that differ significantly from each other ( p < 0.05)

Article Snippet: Subsequently, the cells in one plate were stained with either CD4α-PE (Clone 74–12-4; BD Biosciences, Franklin Lakes, New Jersey, USA) or anti-porcine CD25 (clone K231.3B3; Serotech, Raleigh, NC), followed by washing and staining with an allophycocyanin-conjugated rat anti-mouse IgG1 antibody (Ab) (Clone RMG1–1; Biolegend, San Diego, CA, USA) as a secondary antibody against anti-CD25 according to the manufacturer’s instructions.

Techniques: Derivative Assay, Infection, Expressing

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Pig model mimicking chronic hepatitis E virus infection in immunocompromised patients to assess immune correlates during chronicity

doi: 10.1073/pnas.1705446114

Figure Lengend Snippet: Frequency of CD25+ and CD25− Treg cells at 5, 8, and 13 wpi. The frequency of Treg cells at 5 wpi (A), 8 wpi (B), and 13 wpi (C); of IL-10–secreting Treg cells at 5 wpi (D), 8 wpi (E), and 13 wpi (F); and of TGF-β–secreting Treg cells at 5 wpi (G), 8 wpi (H), and 13 wpi (I) were measured by flow cytometry and expressed as means ± SEM. At each time point, n = 10 pigs, except for the control group (n = 9 pigs). *P < 0.05.

Article Snippet: Briefly, PBMCs (1 × 10 6 cells per well) were first stained at 4 °C for 15 min with FITC-conjugated mouse anti-porcine CD4, Spectral Red-conjugated mouse anti-porcine, and mouse anti-porcine CD25 (IgG1, clone K231.3B2; AbD Serotec), followed by APC-conjugated rat anti-mouse IgG1 (IgG1, clone X56; BD Pharmingen).

Techniques: Flow Cytometry